Application
This unit of competency describes the skills and knowledge to set up a light microscope for optimum resolution, to prepare routine samples and to observe, identify and report sample characteristics.
This unit of competency applies to laboratory or technical assistants in all industry sectors. The unit of competency covers limited interpretation and analysis of results. Troubleshooting of equipment and procedures is not required.
No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Elements and Performance Criteria
Elements describe the essential outcomes. | Performance criteria describe the performance needed to demonstrate achievement of the element. | ||
1 | Prepare samples for examination | 1.1 | Log and label samples according to workplace procedures to ensure traceability |
1.2 | Follow documented standard operating procedures to prepare samples for microscopic examination | ||
2 | Set up and use a light microscope | 2.1 | Carry and use a microscope correctly preventing damage to the equipment and ensuring operator safety |
2.2 | Perform pre-use and safety checks to ensure equipment is fit for purpose | ||
2.3 | Check lenses to ensure they are clean | ||
2.4 | Set up the microscope for optimum resolution and even illumination | ||
2.5 | Select the appropriate objectives and filter for the sample being examined | ||
2.6 | Place sample correctly on the stage | ||
3 | Observe and identify sample characteristics | 3.1 | Recognise and identify significant sample characteristics |
3.2 | Perform required calculations accurately | ||
4 | Maintain a safe work environment | 4.1 | Ensure safety and minimise cross-contamination through the use of personal protective equipment (PPE) |
4.2 | Handle all samples and equipment in accordance with workplace safety protocols | ||
4.4 | Minimise generation of waste and environmental impacts | ||
4.5 | Collect and dispose of all wastes safely | ||
Foundation Skills
This section describes those language, literacy, numeracy and employment skills that are essential to performance.
Numeracy skills to:
perform counts on samples using grids
take basic measurements
perform accurate calculations as required by the method, including:
dilutions
number of cells in original sample after dilution
calculation of cells/ml in a number of squares of a counting chamber
interpret and record test results reliably
log and track samples through all steps from sample receipt to completion of the examination and reporting.
Other foundation skills essential to performance are explicit in the performance criteria of this unit.
Competency Field
Testing